C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/18 (2006.01) C07K 14/475 (2006.01) C12N 15/81 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2746905
The present invention provides the production of recombinant bovine growth hormone (rbGH) using a synthetic gene sequence and maintaining the characteristics of natural bovine growth hormone. According to the present invention, is used a synthetic gene sequence that uses preferential codons and optimized content of nucleotide GC bases for P. pastoris. The present process enables the production of this hormone on a large scale and with high efficiency expression.
L'invention concerne un procédé de production d'hormone de croissance bovine recombinante (rbGH), faisant intervenir une séquence génique synthétique et permettant de maintenir les caractéristiques de l'hormone de croissance bovine naturelle. Selon l'invention, une séquence génique synthétique, qui fait intervenir des codons préférentiels et un contenu optimisé de bases nucléotidiques GC, est utilisée pour P. pastoris. Ce procédé permet de produire cette hormone à grande échelle et avec une efficacité d'expression élevée.
Araripe Goncalves Torres Fernando
Coraucci Neto Dolivar
de Melo Teixeira Marcus
Neiva Santos Suzana
Pepe de Moraes Lidia Maria
Dnatech Projetos Tecnologicos Ltda.
Fundacao Universidade de Brasilia-Fub
Moffat & Co.
Ouro Fino Participacoes E. Empreendimentos S/a.
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