Protecting luciferase activity from luciferase enzyme...

Details Protecting luciferase activity from luciferase enzyme... Protecting luciferase activity from luciferase enzyme...

C - Chemistry, Metallurgy

12

Q

C12Q 1/66 (2006.01) C12N 9/02 (2006.01) C12N 9/96 (2006.01) G01N 21/76 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2508072

A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent.

L'invention concerne un procédé et un kit permettant d'augmenter la tolérance d'un réactif d'analyse aux composés dans un échantillon d'analyse, le réactif d'analyse comprenant une enzyme luciférase. Le procédé consiste à mettre en contact la luciférase avec un agent d'augmentation de tolérance en quantité suffisante pour protéger sensiblement l'activité enzymatique de la luciférase contre l'interférence du composé et de minimiser l'interférence d'au moins environ 10 % par rapport à une analyse dans lequel n'intervient aucun agent d'amélioration de tolérance.

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