Protein disulfide isomerase assay method for the in vitro...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/90 (2006.01) C07K 16/40 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2732676

The present invention relates to a method for the in vitro diagnosis of colorectal cancer by determining the presence of the protein disulfide isomerase tumor marker in a biological sample from a patient suspected of being affected by colorectal cancer through the use of at least one anti-PDI monoclonal antibody against a PDI epitope selected from among the epitopes of sequence SEQ ID NO:1, SEQ ID NO:2 with a similar aromatic amino acid in the three-dimensional PDI structure, and SEQ ID NO:3, said method being useful in the early diagnosis, screening, therapeutic follow-up, prognosis, and relapse diagnosis in the field of colorectal cancer.

La présente invention concerne un procédé de diagnostic in vitro du cancer colorectal par détermination de la présence du marqueur tumoral Protéine Disulfide Isomérase dans un échantillon biologique issu d'un patient suspecté d'être atteint du cancer colorectal en utilisant au moins un anticorps monoclonal anti-PDI dirigé contre un épitope de la PDI choisi parmi les épitopes de séquence SEQ ID N ~1, SEQ ID N~2 avec un acide aminé aromatique proche dans la structure tridimensionnelle de la PDI et SEQ ID N~3, ledit procédé pouvant être utilisé tant dans le diagnostic précoce, le dépistage, le suivi thérapeutique, le pronostic, que dans le diagnostic des rechutes dans le cadre du cancer colorectal.

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