Protein hydrolysates enriched in peptides having a carboxy...

A - Human Necessities – 23 – J

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A23J 3/34 (2006.01) A23J 3/16 (2006.01) A23L 1/29 (2006.01) A23L 1/305 (2006.01) A23L 2/02 (2006.01) A23L 2/84 (2006.01) C12H 1/00 (2006.01) C12N 9/48 (2006.01) C12N 9/62 (2006.01) C12Q 1/37 (2006.01) G01N 33/569 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2437081

A method of enzymatically producing a protein hydrolysate from a protein substrate is described, wherein a proline-specific endoprotease or a composition containing a proline-specific endoprotease and optionally a subtilisin or a metallo endoprotease, and other enzymes such as carboxypeptidases, is used to produce a protein hydrolysate enriched in peptide fragments having a carboxy terminal proline residue. Such protein hydrolysates may be used as such or to reduce bitterness in foods nutritionally supplemented by protein hydrolysates, as well as to produce hydrolysate-containing foodstuffs having low antigenicity.

L'invention concerne un procédé de production par action enzymatique d'un hydrolysat de protéines, à partir d'un substrat de protéines. Selon cette invention, une endoprotéase spécifique de la proline ou une composition contenant une endoprotéase spécifique de la proline, éventuellement une subtilisine ou une métalo-endoprotéase, et d'autres enzymes telles que des carboxypeptidases, est utilisée pour produire un hydrolysat enrichi en fragments peptidiques possédant un reste de proline à terminaison carboxy. De tels hydrolysats de protéines peuvent être utilisés en tant que tels ou pour diminuer l'acidité dans des aliments enrichis d'un point de vue nutritionnel par des hydrolysats de protéines, et pour produire des aliments contenant un hydrolysat et possédant une antigénicité faible.

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