Purified para-nitrobenzyl esterase from bacillus

Details Purified para-nitrobenzyl esterase from bacillus Purified para-nitrobenzyl esterase from bacillus

C - Chemistry, Metallurgy

12

N

C12N 9/18 (2006.01) C12N 15/55 (2006.01) C12P 17/18 (2006.01) C12P 35/00 (2006.01)

Patent

CA 2085803

X-8554 ABSTRACT The invention provides purified PNB esterase from a microorganism of the genus Bacillus. The enzyme is a monomer having a molecular weight of about 54,000 daltons. This PNB esterase catalyzes the de- esterification of PNB-esters of cephalosporin and 1- carbacephalosporin compounds to the free acid form. The invention also provides a process for purifying the enzyme from a Bacillus species. This process comprises a combination of ammonium sulfate fractionation, pH treatment, anion-exchange chromatography, gel filtration, adsorption-desorption chromatography, and affinity chromatography.

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