Purified thermococcus barossii dna polymerase

Details Purified thermococcus barossii dna polymerase Purified thermococcus barossii dna polymerase

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 9/12 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2210895

A preparation of a purified DNA polymerase substantially identical to native DNA polymerase isolated from Thermococcus barossii is disclosed. In a preferable form of the present invention, the DNA polymerase is approximately 68 kDa in size, retains at least 90 % activity after 8 hours of incubation at 95 ~C and retains between 60 % and 80 % proof-reading ability when catalyzing a polymerization reaction with a primer with a 3 base pair mismatch.

Cette invention concerne une préparation d'ADN-polymérase purifiée sensiblement identique à de l'ADN-polymérase double-brin isolée à partir de Thermococcus barossii. Dans un mode de réalisation préféré de la présente invention, l'ADN-polymérase a une taille d'approximativement 68 kDa, conserve au moins 90 % de son activité après 8 heures d'incubation à une température de 95 ·C, et conserve une capacité de correction sur épreuve variant entre 60 et 80 % lors de la catalyse d'une réaction de polymérisation avec une amorce présentant une erreur d'appariement sur 3 paires de base.

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