Quantification of an absorber through a scattering medium

G - Physics – 01 – N

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G01N 21/41 (2006.01) A61B 5/00 (2006.01) A61B 5/1455 (2006.01) A61B 5/20 (2006.01) G01B 11/06 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2741911

There is described a method for determining a quantitative parameter of a compound in an analysis sample, comprising: providing a scattering medium in physical contact with the analysis sample, the scattering medium having at least one layer, an index of refraction of the scattering medium being superior to an index of refraction of the analysis sample; propagating, in the scattering medium, an incident beam of light having a wavelength substantially corresponding to an absorption wavelength of the compound such that an evanescent wave is generated at an interface between the scattering medium and the analysis sample; taking n intensity measurements of a reflected beam of light for the analysis sample, n being superior to one; and determining the quantitative parameter of the compound using the n intensity measurements for the analysis sample.

L'invention concerne un procédé permettant de déterminer un paramètre quantitatif d'un composé contenu dans un échantillon d'analyse. Ce procédé consiste à : mettre à disposition un milieu de dispersion en contact physique avec l'échantillon d'analyse, le milieu de dispersion présentant au moins une couche, l'indice de réfraction du milieu de dispersion étant supérieur à l'indice de réfraction de l'échantillon d'analyse; propager, dans le milieu de dispersion, un faisceau incident de lumière présentant une longueur d'onde correspondant sensiblement à une longueur d'onde d'absorption du composé de sorte qu'une onde évanescente est générée au niveau d'une interface située entre le milieu de dispersion et l'échantillon d'analyse; prendre n mesures d'intensité d'un faisceau de lumière réfléchi pour l'échantillon d'analyse, n étant supérieur à 1, et déterminer, pour l'échantillon d'analyse, le paramètre quantitatif du composé en fonction des n mesures d'intensité.

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