Real-time sequence determination

Details Real-time sequence determination Real-time sequence determination

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12M 1/00 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 15/09 (2006.01) G01N 21/64 (2006.01) G01N 21/78 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2415897

A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.

L'invention concerne une méthodologie de séquençage direct, sensiblement en temps réel, de molécule d'ADN ou d'ARN unique ou bien d'une partie de cette molécule. On procède par manipulation de polymérase et/ou de dNTP avec des étiquettes atomiques et/ou moléculaires susceptibles d'être détectées sur la base d'une propriété elle-même contrôlée par un système de détection.

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