Recombinant collagen and derived proteins produced by...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/00 (2006.01) A01H 5/00 (2006.01) C07K 14/78 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2274807

The invention concerns the use of a recombinant nucleotide sequence containing a DNAc coding for one or several mammal collagen chains or derived proteins and elements enabling a plant cell to produce the collagen chain(s) or derived proteins, coded by said DNAc, particularly a transcription promoter and terminator identified by the transcription machinery of the plant cells, for transforming the plant cells so as to obtain from these cells, or plants obtained from them, the collagen chain(s) or derived proteins. The invention also concerns the resulting proteins and transformed plant material as well as their uses.

L'invention a pour objet l'utilisation d'une séquence nucléotidique recombinante contenant, d'une part, un ADNc codant pour une ou plusieurs chaînes de collagène de mammifères ou les protéines dérivées, et d'autre part, les éléments permettant à une cellule végétale de produire la ou les chaînes de collagène ou les protéines dérivées, codées par ledit ADNc, notamment un promoteur et un terminateur de transcription reconnus par la machinerie transcriptionnelle des cellules végétales, pour la transformation de cellules de plantes en vue de l'obtention, à partir de ces cellules, ou de plantes obtenues à partir de ces dernières, de la ou des chaînes de collagène ou des protéines dérivées; les protéines et le matériel végétal transformé ainsi obtenus ainsi que leurs utilisations.

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