Recombinant deamidated gliadin antigen

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) A61K 38/00 (2006.01) C07K 14/415 (2006.01) C07K 19/00 (2006.01) C12N 15/63 (2006.01) G01N 33/53 (2006.01) G01N 33/564 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2721582

The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

La présente invention concerne un procédé permettant de déterminer si un sujet souffre de maladie cliaque en mettant en contact un échantillon de fluide corporel prélevé sur le sujet et un antigène constitué dune protéine de fusion de gliadine immobilisée sur un support solide. La protéine de fusion de gliadine de lantigène contient une gliadine recombinante déamidée liée à un marqueur tel que la protéine glutathione-S transférase (GST). Lantigène est préparé par immobilisation de la protéine de fusion de gliadine sur le support solide par lintermédiaire du marqueur. Lantigène peut en outre inclure un tissu transglutaminase (tTG) réticulé avec la protéine de fusion de gliadine. Lorsquun tTG est présent, ledit tTG et la gliadine recombinante déamidée sont mélangés avant limmobilisation en phase solide.

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