Recombinant vector containing a lipoprotein gene sequence...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/74 (2006.01) A61K 39/002 (2006.01) A61K 39/29 (2006.01) A61K 39/395 (2006.01) C07K 14/02 (2006.01) C07K 14/21 (2006.01) C07K 14/44 (2006.01) C07K 14/445 (2006.01) C07K 14/455 (2006.01) C07K 16/12 (2006.01) C07K 16/20 (2006.01) C07K 16/42 (2006.01) C07K 16/46 (2006.01) C07K 19/00 (2006.01) C12N 15/13 (2006.01) C12N 15/30 (2006.01) C12N 15/31 (2006.01) C12N 15/51 (2006.01) C12N 15/62 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2168426

A recombinant vector for cloning a heterologous nucleotide sequence and/or expressing it and/or transferring it to a cell host. The vector includes, at a site which is not essential for replication, the gene coding for a lipoprotein other than E. Coli lipoproteins, or a part of said gene which contains the elements required for controlling the expression of said lipoprotein and exposing it on the surface of the outer host cell membrane, so that the heterologous nucleotide sequence can be inserted into the gene or said part thereof under conditions suitable for expressing said heterologous sequence and exposing it on the surface of the cell host.

L'invention a pour objet un vecteur recombinant pour le clonage et/ou l'expression et/ou le transfert dans un hôte cellulaire d'une séquence de nucléotides hétérologue, caractérisé en ce qu'il comprend, en un site non essentiel pour sa réplication, le gène codant pour une lipoprotéine différente des lipoprotéines de Ecoli, ou une partie de ce gène contenant des éléments nécessaires au contrôle de l'expression de cette lipoprotéine et à son exposition à la surface de la membrane cellulaire externe de l'hôte, de façon telle que la séquence de nucléotides hétérologue est susceptible d'être introduite dans le gène ou la partie du gène codant pour la lipoprotéine, dans des conditions permettant l'expression de cette séquence hétérologue et son exposition à la surface de l'hôte cellulaire.

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