C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C12N 1/04 (2006.01) C12N 1/19 (2006.01) C12N 9/14 (2006.01) C12N 15/55 (2006.01) C12N 15/63 (2006.01) C12P 17/02 (2006.01) C12P 21/02 (2006.01) C12P 41/00 (2006.01)
Patent
CA 2604917
The invention provides isolated Y. lipolytica cells and substantially pure cultures of Y. lipolytica cells containing exogenous nucleic acids encoding EH polypeptides, e.g., enantioselective EH polypeptides. Also featured by the invention are methods for the production of the EH polypeptides and methods for hydrolysing epoxides and for producing optically active vicinal diols and/or optically active epoxides. Also embodied by the invention are efficient integrative expression vectors.
L'invention concerne des cellules d'Y. lipolytica isolées et des cultures de cellules d'Y. lipolytica sensiblement pures contenant des acides nucléiques exogènes codant pour des polypeptides EH, par exemple, des polypeptides EH énantio-sélectifs. L'invention concerne également des procédés permettant de produire lesdits polypeptides EH et des procédés permettant d'effectuer l'hydrolyse des époxydes et de produire des dols vicinaux et/ou des époxydes optiquement actifs. L'invention concerne enfin des vecteurs d'expression intégratifs efficaces.
Botes Adriana Leonora
Labuscagne Michel
Lalloo Rajesh
Lotter Jeanette
Mitra Robin Kumar
Csir
Gowling Lafleur Henderson Llp
Oxyrane Uk Limited
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