Recombinantly expressed carboxypeptidase b and purification...

Details Recombinantly expressed carboxypeptidase b and purification... Recombinantly expressed carboxypeptidase b and purification...

C - Chemistry, Metallurgy

12

N

C12N 9/48 (2006.01)

Patent

CA 2486195

The invention provides a method to produce a protein with carboxypeptidase B activity from a pro-carboxypeptidase B zymogen, derived from a non-animal host organism. Carboxypeptidase B is activated from the zymogen using non- denaturing conditions. Particularly, the activation is performed under conditions that avoid unwanted non-covalent binding of the propeptide to the activated carboxypeptidase B enzyme.

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