Rps gene family, primers, probes, and detection methods

Details Rps gene family, primers, probes, and detection methods Rps gene family, primers, probes, and detection methods

C - Chemistry, Metallurgy

12

N

C12N 15/29 (2006.01) C07H 21/00 (2006.01) C07K 14/415 (2006.01) C07K 16/16 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2187771

Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.

L'invention porte sur de l'ADN sensiblement pur codant le polypeptide Arabidopsis thaliana Rps2, sur ledit polypeptide Rps2 sensiblement pur et sur des méthodes d'utilisation dudit ADN pour exprimer ledit polypeptide Rps2 dans des cellules végétales et des plantes en vue de l'obtention de plantes transgéniques résistantes aux germes pathogènes. L'invention porte également sur les caractéristiques de régions conservées de la famille RPS, d'amorces et d'ondes servant à identifier et isoler d'autres gènes RPS de résistance aux maladies.

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