Sequential protein isolation and purification schemes by...

A - Human Necessities – 23 – J

Patent

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A23J 1/06 (2006.01) A61K 35/14 (2006.01) A61K 35/16 (2006.01) C07K 1/22 (2006.01) C07K 17/00 (2006.01)

Patent

CA 2576963

The invention discloses methods for sequential protein isolation and purification from a biological sample by affinity chromatography. Affinity chromatography is conducted using ligands or ligand support complexes that selectively and specifically bind to proteins in the biological sample. The ligands or ligand support complexes were contacted sequentially in a predetermined order with the biological sample to allow each ligand or ligand- support complex to sequentially bind a protein from the biological sample.

L'invention porte sur des procédés d'isolement et de purification séquentiels de protéines d'un prélèvement biologique par chromatographie d'affinité. On procède à la chromatographie d'affinité au moyen de ligands ou de complexes de support de ligands qui se lient sélectivement et spécifiquement aux protéines du prélèvement biologique. Les ligands ou les complexes de support de ligands sont mis en contact séquentiellement selon un ordre prédéterminé avec le prélèvement biologique afin de permettre à chaque ligand ou complexe de support de ligand de se lier séquentiellement à une protéine du prélèvement biologique.

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