C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C12N 9/64 (2006.01)
Patent
CA 2546451
The present invention provides novel serine proteases, novel genetic material encoding these enzymes, and proteolytic proteins obtained from Micrococcineae spp., including but not limited to Cellulomonas spp. and variant proteins developed therefrom. In particular, the present invention provides protease compositions obtained from a Cellulomonas spp, DNA encoding the protease, vectors comprising the DNA encoding the protease, host cells transformed with the vector DNA, and an enzyme produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising protease(s) obtained from a Micrococcineae spp., including but not limited to Cellulomonas spp. In alternative embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These mutant proteases also find use in numerous applications.
La présente invention concerne de nouvelles sérine protéases, de nouvelles matières génétiques codantes pour ces enzymes, et des protéines protéolytiques obtenues à partir de Micrococcineae spp., y compris de manière non exhaustive de Cellulomonas spp. et les variantes protéiques développées à partir de ces derniers. L'invention concerne notamment des compositions de protéases obtenues à partir de Cellulomonas spp, l'ADN codant pour la protéase, les vecteurs contenant le NA codant pour la protéase, les cellules hôtes transformées par le vecteur ADN, et un enzyme produit par les cellules hôtes. La présente invention concerne également des compositions de nettoyage (notamment des compositions de détergents), des compositions d'aliments pour animaux, et des compositions de traitement du textile et du cuir contenant des protéase(s) obtenues à partir du Micrococcineae spp., y compris de manière non exhaustive le Cellulomonas spp. En variante, la présente invention concerne des protéases mutantes (notamment variantes) dérivées des protéases de type sauvage. Les domaines d'applications de ces protéases mutantes sont également variés.
Jones Brian Edward
Kolkman Marc
Leeflang Chris
Oh Hiroshi
Poulose Ayrookaran J.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor International Inc.
The Procter & Gamble Company
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