Single chain antigen-binding polypeptides for polymer...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/13 (2006.01) A61K 39/395 (2006.01) C07K 16/24 (2006.01) C07K 16/46 (2006.01) C12N 15/63 (2006.01) C12P 21/08 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2521162

The present invention relates to monovalent and multivalent single-~chain antigen-binding polypeptides with site-specific modifications. The provided polypeptides are capable of being covalently linked or conjugated to polyalkylene oxides at the modified sites. The resulting conjugates retain antigen binding properties and exhibit prolonged circulating time and reduced antigenicity relative to unconjugated single chain antigen binding polypeptides. Methods and compositions for making and using the single chain antigen-binding polypeptides with site-specific modifications are also provided.

L'invention concerne des polypeptides de liaison d'antigène à chaîne simple monovalents et multivalents, comprenant des modifications spécifiques de site. Les polypeptides de l'invention peuvent être liés de manière covalente ou conjugués à des oxydes de polyalkylène sur les sites modifiés. Les conjugués ainsi obtenus conservent les propriétés de liaison de l'antigène et présentent une durée de circulation prolongée et une antigénécité réduite par rapport à un polypeptide de liaison d'antigène à chaîne simple. L'invention concerne également des procédés et des compositions pour fabriquer et utiliser les polypeptides de liaison d'antigène à chaîne simple à modifications spécifiques de sites.

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