C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/00 (2006.01) C12N 1/20 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2428999
A solid culture medium exhibiting a 10-minute average rate of water absorption of at least 0.05 ml/min, which is prepared by a method for preparation, characterized in that it comprises dissolving respective components of the solid culture medium other than a dissolving water into the dissolving water, solidifying the resultant solution, drying the solidified culture medium to remove water, wherein the water is removed in an amount such that the solid culture medium after the removal of water exhibits a 10-minute average rate of water absorption of at least 0.05 ml/min, wherein the 10-minute-average water absorption rate is calculated for an area of the medium which is about 58.1 cm2 with a weight of about 15 gm, and the amount of the dissolving water is more than an amount specific for the medium by approximately the same amount as that of the water removed. The solid culture medium is free from problems relating to the inhibition of growth of microbes due to a dried condition, exhibits an enhanced amount of samples in a short time, and is thus significantly more suitable for rapid and accurate measurement of the colony count for microbes.
La présente invention concerne un milieu de culture solide capable d'absorber au moins 0,05 ml d'eau par minute pendant 10 minutes. Ce milieu s'élabore selon un procédé faisant intervenir une dissolution dans l'eau des différents composants non aqueux du milieu de culture solide, puis une solidification de la solution obtenue, et un séchage du milieu de culture solide pour le déshydrater. En l'occurrence, on pousse la déshydratation au point que le milieu de culture solide ainsi déshydraté devient capable d'absorber au moins 0,05 ml d'eau par minute pendant 10 minutes. En outre, la quantité d'eau de solution est supérieure à la teneur spécifique du milieu d'une proportion égale à environ la quantité d'eau éliminée par déshydratation. Ce milieu de culture solide ne donne lieu à aucun problème de blocage de croissance des microbes malgré l'état de déshydratation, et permet une plus grande quantité d'échantillons en un temps très court, ce qui en fait un milieu particulièrement approprié pour l'évaluation rapide et précise de la numération microbienne des colonies.
Fukuwatari Yasuo
Karino Kenichiro
Kiyotaki Kenji
Nakagawa Minoru
Sasaki Kazue
Morinaga Milk Industry Co. Ltd.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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