Soluble canola protein isolate production from protein...

A - Human Necessities – 23 – J

Patent

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A23J 1/14 (2006.01) A23J 3/14 (2006.01) A23K 1/14 (2006.01) A23L 1/305 (2006.01) A23L 2/66 (2006.01) C07K 1/00 (2006.01) C07K 1/34 (2006.01) C07K 14/415 (2006.01)

Patent

CA 2731681

A soluble canola protein isolate is prepared from canola protein micellar mass by solubilizing the protein micellar mass in a calcium salt solution, preferably a calcium chloride solution, followed by dilution of the resulting canola protein solution. Following removal of the precipitate phytic acid, the aqueous canola protein solution is concentrated, optionally diafiltered, and acidified to a pH of about 2.5 to 4.0 to produce an acidified clear canola protein solution, which may be concentrated, subjected to a colour removal step and dried. The canola protein isolate so formed is soluble, transparent and heat stable in an acid aqueous environment and also is soluble at natural pH, without precipitation of protein.

Un isolat de protéine de canola soluble est préparé à partir d'une masse micellaire de protéines en solubilisant la masse micellaire de protéines dans une solution de sel de calcium, de préférence une solution de chlorure de calcium, suivie d'une dilution de la solution de protéines de canola résultante. Après élimination de l'acide phytique précipité, la solution aqueuse de protéines de canola est concentrée, éventuellement diafiltrée, et acidifiée jusqu'à un pH d'environ 2,5 à 4,0 afin de produire une solution acidifiée claire de protéines de canola, qui peut être concentrée, soumise à une étape d'élimination de la couleur et séchée. L'isolat de protéines de canola ainsi formé est soluble, transparent et stable à la chaleur dans un environnement aqueux acide et est également soluble à pH neutre, sans précipitation de protéines.

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