Sorbitol dehydrogenase, gene encoding the same and use thereof

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/53 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12P 7/60 (2006.01) C12P 19/02 (2006.01)

Patent

CA 2365092

A gene encoding D-sorbitol dehydrogenase (SLDH); a process for producing SLDH by culturing host cells transformed by an expression vector having the above gene; and a process for producing L-sorbose or 2-keto-L-gulonic acid (2KLGA) by using the above culture. 2KLGA is an important intermediate in the production of L-ascorbic acid. Thus, a process for producing L-ascorbic acid from the 2KLGA obtained by the above process is also provided.

L'invention concerne un gène codant pour la D-sorbitol déshydrogénase (SLDH); un procédé de production de SLDH par la mise en culture de cellules hôtes transformées au moyen d'un vecteur d'expression possédant ledit gène; et un procédé de production de L-sorbose ou d'acide 2-céto-L-gulonique (2KLGA) au moyen de ladite culture. 2KLGA est un intermédiaire important dans la production d'acide L-ascorbique. Par conséquent, l'invention concerne également un procédé de production d'acide L-ascorbique à partir de 2KLGA obtenu à l'aide procédé mentionné.

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