C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C07H 19/06 (2006.01) C07H 19/16 (2006.01)
Patent
CA 2277198
The invention relates to stable aqueous solutions containing one or more nucleoside triphosphates, wherein each solution has a pH value higher than 7.5 and no additional stabilizing substances. The nucleoside triphosphate solutions are specially used for DNA synthesizing reactions, for instance Rt-PCR, cycle sequencing, random priming and nick translation. One of the most important applications of corresponding solutions containing deoxy-nucleoside triphosphate (d-NTP) is their use in the polymerase chain reaction (PCR).
L'invention concerne des solutions aqueuses stabilisées renfermant un ou plusieurs triphophates de nucléosides, chacune de ces solutions ayant un pH supérieur à 7,5 et ne renfermant aucune substance stabilisante supplémentaire. Les solutions de triphosphates de nucléosides sont utilisées notamment pour des réactions de synthèse de l'ADN, telles que, par exemple, RT-PCR, séquençage de cycle, amorçage aléatoire et translation de coupure. L'une des applications les plus importantes des solutions correspondantes renfermant du désoxy-nucléoside-triphosphate (d-NTP) est son utilisation dans la réaction en chaîne de la polymérase (PCR).
Ihlenfeldt Hans-Georg
Leitenberger Volker
Muhlegger Klaus
Schmidt Axel
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Roche Diagnostics Gmbh
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