Stereoselective reductive amination of ketones

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 7/00 (2006.01) C12N 9/06 (2006.01) C12P 7/62 (2006.01) C12P 17/00 (2006.01) C12P 17/02 (2006.01) C12P 17/04 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2337230

Processes for stereoselective enzymatic conversion of certain keto carboxylic acid derivatives to form the corresponding alkylamino acid compounds are described. The invention also concerns an engineered yeast host cell containing recombinant nucleic acid capable of expressing a phenylalanine dehydrogenase, as well as an engineered host cell containing recombinant nucleic acid capable of expressing a phenylalanine dehydrogenase enzyme and nucleic acid capable of expressing a formate dehydrogenase enzyme.

L'invention porte sur des processus de conversion enzymatique stéréosélective de certains dérivés d'acide céto carboxylique permettant d'obtenir les composés correspondants d'acide alkylamino. Cette invention porte également sur une cellule hôte de levure mise au point et contenant un acide nucléique de recombinaison capable d'exprimer une déshydrogénase de phénylalanine, ainsi que sur une cellule hôte mise au point contenant un acide nucléique de recombinaison capable d'exprimer une enzyme déshydrogénase de phénylalanine et un acide nucléique capable d'exprimer une enzyme déshydrogénase formatée.

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