Streptomyces avermitilis gene directing the ratio of b2:b1...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/60 (2006.01) C12N 1/21 (2006.01) C12N 9/88 (2006.01) C12N 15/31 (2006.01) C12N 15/76 (2006.01) C12P 17/18 (2006.01) C12P 19/62 (2006.01)

Patent

CA 2475214

The present invention relates to polynucleotide comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

L'invention porte sur des polynucléotide comprenant des séquences nucléotidiques codant pour le produit génique du aveC, lesdites molécules de polynucléotide pouvant servir à modifier le rapport entre les classes 2 et 1, ou la quantité, des avermectines produites dans des cultures de fermentation du S. avermitis. L'invention porte également sur des vecteurs, des cellules hôtes, et des souches de mutants du S. avermitis dans lesquelles le gène du aveC a été inactivé ou muté afin de modifier le rapport entre les classes 2 et 1, ou la quantité, des avermectines produites.

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