Synthetic heparanase molecules and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/62 (2006.01) C12N 15/866 (2006.01)

Patent

CA 2537363

The present invention relates to synthetically produced, enzymatically active heparanase nucleic acid molecules that are capable of expression in high yield heterologous expression systems, and to polypeptides encoded by said molecules. Also provided herein are methods of expressing mammalian heparanase in heterologous expression systems, wherein high yields of biologically active heparanase are produced compared to prior art methods.

Cette invention concerne des molécules d'acide nucléique d'héparanase à activité enzymatique produites par synthèse, lesquelles molécules peuvent être exprimées dans des systèmes d'expression hétérologues à haut rendement et concerne également des polypeptides codés par ces molécules. Cette invention concerne en outre des procédés permettant d'exprimer l'héparanase mammifère dans des systèmes d'expression hétérologues. Ces procédés, comparés aux procédés de la technique antérieure, permettent d'obtenir une production à haut rendement d'héparanase biologiquement active.

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