C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/00 (2006.01) C12N 5/071 (2010.01) C07K 16/00 (2006.01) C07K 16/28 (2006.01) C07K 16/46 (2006.01)
Patent
CA 2388245
The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2/3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2/0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2/0 has a high antibody-dependent cell- mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.
L'invention concerne un procédé de production d'un polypeptide voulu par l'utilisation de cellules de rat. Plus particulièrement, l'invention concerne un procédé de production du polypeptide consistant en la mise en culture de cellules de rat telles que YB2/3HL.P2.G11.16Ag.20 (ci-après dénommé YB2/0), de préférence des cellules de rat obtenues par transfert d'un ADN recombiné contenant un ADN codant le polypeptide voulu tel qu'une molécule à fonctionnalité immunologique, dans un milieu sans sérum. Parmi les polypeptides voulus obtenus selon cette méthode, un anticorps obtenu, par exemple, à l'aide de transformants de YB2/0 présente une haute activité cytotoxique dépendante des anticorps et est par conséquent utile sous forme de médicaments.
Akashi Naohisa
Konno Yoshinobu
Ogawa Tatsuya
Sugimoto Seiji
Takasugi Hiroshi
Goudreau Gage Dubuc
Kyowa Hakko Kirin Co. Ltd.
Kyowa Hakko Kogyo Co. Ltd.
LandOfFree
The use of serum-free adapted rat cells for producing... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with The use of serum-free adapted rat cells for producing..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and The use of serum-free adapted rat cells for producing... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1936565