Thermal cycler for pcr

G - Physics – 01 – N

Patent

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Details

G01N 35/00 (2006.01)

Patent

CA 2464973

An instrument for performing highly accurate PCR employing an assembly, a heated cover and an internal computer. The assembly is made up of a sample block, a number of Peltier thermal electric devices and heat sink, clamped together. The sample block temperature is changed exclusively by the thermoelectric devices (39) controlled by the computer. The sample block is of low thermal mass and is constructed of silver. The Pettier devices are designed to provide fast temperature excursions over a wide range. The heat sink has a perimeter trench to minimize edge losses and is adjacent to a continuously variable fan. A perimeter heater is used to improve the thermal unifomity across the sample block to approximately +/- 0.2°C.

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