Thermophilic organisms for conversion of lignocellulosic...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/06 (2006.01)

Patent

CA 2651011

Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALKl has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALKl, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

La présente invention concerne des organismes thermophiles mutants qui consomment divers substrats dérivés de la biomasse. L'invention concerne des souches de Thermoanaerobacterium saccharolyticum chez lesquelles l'expression de l'acétate kinase et de la phosphotransacétylase a été éliminée. En outre, la souche ALKl a été fabriquée par recombinaison homologue dirigée pour inhiber la production de l'acide acétique et de l'acide lactique. Une culture en continu mettant en jeu une provocation basée sur la concentration en substrat a conduit à l'évolution de ALKl et à la formation d'une souche plus robuste appelée ALK2. Les organismes peuvent être utilisés par exemple dans des réactions SSF et SSCF thermophiles réalisées à des températures qui sont optimales pour l'activité cellulase afin de produire de l'éthanol à des rendements proches du rendement théorique sans exprimer la pyruvate décarboxylase.

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