Use of an avirulent bordetella mutant as a live vaccine vector

A - Human Necessities – 61 – K

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Details

A61K 39/10 (2006.01) A61K 39/275 (2006.01) C12N 7/01 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2678698

The present invention pertains to Bordetella bacteria having a double mutation, a first mutation in a gene of the Type III secretion system and a second mutation in a gene of the adenylate cyclase toxin (CyaA) locus of the bacteria so that the mutations result in no Type III secretion system, a non-functional Type III secretion system, no CyaA protein, or a non-functional CyaA protein or a combination thereof. The Bordetella bacteria double mutant is attenuated while maintaining the efficacy of the bacteria to elicit an immune response. The present invention also pertains to vaccine compositions and methods for treating and immunizing a mammal against a disease caused by infection of Bordetella bacteria or a disease caused by a pathogen.

Cette invention concerne une bactérie Bordetella à double mutation, la première dans un gène du système de sécrétion de type III, et la seconde dans un gène du locus de la toxine adénylase cyclase (CyaA) de la bactérie de sorte que ces mutations ne se débouchent pas sur un système de sécrétion de Type III, mais sur un système de sécrétion de Type III non fonctionnel, non pas sur une protéine CyaA, mais sur une protéine CyaA non fonctionnelle ou une combinaison de ces dernières. Le double mutant de la bactérie Bordetella est atténué tout en conservant l'efficacité de la bactérie dans le déclenchement d'une réponse immunitaire. L'invention concerne également des compositions de vaccin contre une maladie provoquée par une infection de la bactérie Bordetella ou par un agent pathogène

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