Vectors and methods for expression of human protein c activity

Details Vectors and methods for expression of human protein c activity Vectors and methods for expression of human protein c activity

C - Chemistry, Metallurgy

12

N

530/13, 195/1.22

C12N 15/12 (2006.01) C07H 21/00 (2006.01) C07K 14/47 (2006.01) C12N 5/10 (2006.01) C12N 9/64 (2006.01) C12N 15/70 (2006.01) C12N 15/79 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1341073

The present invention relates to DNA compounds which encode human protein C activity. A variety of eukaryotic and prokaryotic recombinant DNA expression vectors have teen constructed that comprise the novel protein C activity-encoding DNA and drive expression of protein C activity when transformed into an appropriate host cell. The novel expression vectors can be used to produce protein C derivatives, such as non-carboxylated, non-glycosylated, or non-hydroxylated protein C, and to produce protein C precursors, such as nascent or zymogen protein C, and to produce subfragments of protein C, such as actives or inactive light and heavy chain. The recombinant-produced protein C activity is useful in the treatment and prevention of a variety of vascular disorders.

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