Virus-like particles as vaccines for paramyxovirus

A - Human Necessities – 61 – K

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A61K 39/155 (2006.01) A61K 39/12 (2006.01)

Patent

CA 2617508

The present invention discloses the method of making and using a novel, non- infective, paramyxovirus vaccine. Paramyxovirus structural proteins within a virus-like particle (VLP) comprise one example of such a vaccine. It is observed that the presence of matrix protein, alone, is sufficient and necessary to provide an effective VLP release. Co-expression of four paramyxovirus structural proteins, however, result in the release of non- infective VLPs with densities and efficiencies of release similar to that of infective particles. Representative diseases wherein a VLP vaccine might be useful include, but are not limited to, Newcastle disease, measles, respiratory syncytial virus infection, and parainfluenza 3 virus infection.

La présente invention concerne un procédé de fabrication et d'utilisation d'un nouveau vaccin contre le paramyxovirus non infectieux. Les protéines structurales du paramyxovirus dans une pseudo-particule virale (VLP) comprennent un exemple de ce vaccin. On a observé que la présence d'une protéine matrice isolée est suffisante et nécessaire pour obtenir une libération efficace de VLP. Toutefois, la co-expression de quatre protéines structurales du paramyxovirus implique la libération de VLP non infectieuses présentant des densités et des efficacités de libération similaires à celles des particules infectieuses. Les maladies représentatives dans lesquelles un vaccin VLP pourrait être utile incluent, entre autres, la maladie de Newcastle, la rougeole, l'infection par le virus respiratoire syncytial et l'infection par le virus de parainfluenza de type 3.

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