Yeast cells expressing an exogenous cellulosome and methods...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/10 (2006.01) C07K 14/33 (2006.01) C12N 9/42 (2006.01) C12N 15/56 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2752976

The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

La présente invention porte sur l'ingénierie génétique et l'expression de cellulosomes hétérologues dans des microorganismes afin de faciliter la conversion de biomasse en des produits utiles. Dans certains modes de réalisation, l'invention porte sur l'expression de protéines d'échafaudage qui forment le noyau d'un cellulosome. Des cellulases ou autres enzymes de dégradation de biomasse peuvent être liés de façon non covalente à la protéine d'échafaudage en vertu d'une interaction domaine dockérine-domaine cohésine.

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