Zymomonas with improved ethanol production in medium...

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 7/06 (2006.01) C07K 14/195 (2006.01) C12N 1/00 (2006.01) C12N 1/21 (2006.01) C12N 1/38 (2006.01) C12N 9/10 (2006.01) C12N 9/92 (2006.01) C12N 15/52 (2006.01) C12N 15/63 (2006.01) C12N 15/74 (2006.01) C12P 7/10 (2006.01)

Patent

CA 2704532

Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himAgene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.

L'invention concerne le criblage d'une bibliothèque de mutants de Zymomonas dans lequel le gène himA s'est avéré être impliqué dans l'effet inhibiteur de l'acétate sur la performance de Zymomonas. Du Zymomonas utilisant du xylose davantage modifié pour réduire l'activité du gène himA s'est avéré avoir une production accrue d'éthanol comparé à une souche parente, une fois mis en culture dans un milieu comprenant du xylose et de l'acétate.

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