Process for the recovery of .alpha.-lactalbumin and...

A - Human Necessities – 23 – J

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A23J 1/20 (2006.01) A23C 9/146 (2006.01) C07K 1/14 (2006.01) C07K 14/435 (2006.01)

Patent

CA 2111668

16 ABSTRACT Title: Process for the recovery of .alpha.-lactalbumin and .beta.-lactoglobulin from a whey protein product This invention relates to a process for the recovery of .alpha.-lactalbumin and/or .beta.-lactoglobulin enriched whey protein concentrate from a whey protein product. The process comprises the following steps: a) incubating a solution comprising said whey protein product with a calcium-binding ionic exchange resin in its acid form to initiate the instabilization of .alpha.-lactalbumin, b) adjusting the pH of the treated protein product solution to a value between 4.3 and 4.8, after separation of said resin, c) incubating said protein product solution at a temperature between 10 and 50°C to promote the flocculation of .alpha.-lactalbumin, d) fractionating the proteins in said protein product solution at pH 4.3-4.8, providing an .alpha.-lactalbumin enriched fraction and a .beta.-lactoglobulin enriched fraction, e) raising the pH of the .alpha.-lactalbumin enriched fraction sufficiently to solubilize the .alpha.-lactalbumin fraction, and f) optionally raising the pH of the .beta.-lactoglobulin enriched fraction sufficiently to neutralize the .beta.-lactoglobulin fraction.

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