Hybridization-triggered fluorescent detection of nucleic acids

Details Hybridization-triggered fluorescent detection of nucleic acids Hybridization-triggered fluorescent detection of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 19/00 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2387084

Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properties of the compositions change in a detectable manner upon hybridization to a nucleic acid containing a target sequence. Methods for synthesis and methods of use of the compositions are also provided.

L'invention concerne des compositions et des méthodes de détection d'acides nucléiques par fluorescence. Ces compositions peuvent être détectées par fluorescence lorsqu'elles sont hybridées à un acide nucléique contenant une séquence cible, mais n'ont pas de pouvoir fluorescent à l'état non hybridé. Dans une autre forme de réalisation, le pouvoir fluorescent des compositions change de façon visible une fois ces compositions hybridées à un acide nucléique contenant une séquence cible. L'invention concerne en outre des méthodes de synthèse et des méthodes d'utilisation desdites compositions.

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