Chromatographic separation method of paclitaxel and...

C - Chemistry – Metallurgy – 07 – D

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C07D 305/14 (2006.01)

Patent

CA 2398405

A method for separating paclitaxel from cephalomannin and other related compounds by obtaining a starting material that contains paclitaxel and cephalomannin; dissolving the starting material in any one of a number of particularly defined solvents such as butyl formate and butyl acetate to form a mixture; subjecting the mixture to column chromatography to obtain an eluted fraction of paclitaxel, an eluted fraction of cephalomannin and a residue; and separately drying the paclitaxel and cephalomannin fractions to obtain separate crystalline forms of paclitaxel and cephalomannin, respectively.

La présente invention concerne un procédé qui permet de séparer le paclitaxel, la céphalomannine et d'autres composés liés de la manière suivante : on obtient un produit de départ contenant du paclitaxel et de la céphalomannine ; on dissout le produit de départ en un quelconque nombre de solvants spécifiquement définis tels que le formate de butyle et l'acétate de butyle afin de former un mélange ; on soumet le mélange à une chromatographie en colonnes afin d'obtenir une fraction éluée de paclitaxel et de céphalomannine et un résidu ; et on dessèche séparément les fractions de paclitaxel et de céphalomannine afin d'obtenir des formes cristallines de paclitaxel et de céphalomannine, respectivement.

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