Method for purifying gbs toxin/cm101

Details Method for purifying gbs toxin/cm101 Method for purifying gbs toxin/cm101

C - Chemistry, Metallurgy

08

B

C08B 37/00 (2006.01) A61K 31/715 (2006.01) C12P 1/04 (2006.01) C12P 19/26 (2006.01)

Patent

CA 2265696

A method for purifying a polysaccharide from group B .beta.-hemolytic Streptococcus (GBS) bacteria includes contacting a bacterial fermentation stock with a hydrophobic interaction chromatography (HIC) resin. Additional steps may include a phenol/saline extraction and an ion exchange chromatography. The method results in a product having very high purity. The product of the purification provides a composition which is highly useful in both research and therapeutic settings.

Cette invention se rapporte à un procédé de purification d'un polysaccharide de bactéries du type Streptococcus- beta hémolytique du groupe B (GBS). Ledit procédé consiste à mettre en contact une matière de fermentation bactérienne avec une résine pour chromatographie hydrophobe (HIC). Il consiste également à effectuer une extraction dans une solution saline contenant du phénol et une chromatographie par échange d'ions. Ledit procédé permet d'obtenir un produit de très grande pureté. Ce produit de la purification constitue une composition extrêmement utile à la fois à la recherche et en environnement thérapeutique.

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