Purification of q beta replicase

Details Purification of q beta replicase Purification of q beta replicase

C - Chemistry, Metallurgy

12

N

195/78, 195/33.1

C12N 9/16 (2006.01) C12N 9/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2018566

PURIFICATION OF Q BETA REPLICASE Abstract of the Disclosure A process of producing highly pure, Q Beta replicase having a high level of activity is described. The present process allows isolation of Q Beta replicase from recombinant bacteria containing a clone of a phage DNA encoding the 65,000 weight subunit of Q Beta replicase. The present process provides an efficient method for producing pure Q Beta replicase which can readily be scaled up to commercial production levels.

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