Process for the production of biological substances by...

Details Process for the production of biological substances by... Process for the production of biological substances by...

C - Chemistry, Metallurgy

12

P

C12P 1/00 (2006.01) C12M 3/00 (2006.01) C12N 1/02 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2523792

The invention relates to a process for the production of a biological substance by perfusion culturing of suspended animal cells in a serum free cell culture medium, wherein the biological substance is separated from the cells by filtration, characterized in that at least 0.001w/w% nonionic surfactant is present in the cell culture medium. It has been found that the presence of nonionic surfactant in the cell culture medium improves the filter performance.

L'invention concerne un processus destiné à la fabrication d'une substance biologique par la culture sous perfusion de cellules animales suspendues dans un milieu de culture exempt de sérum dans lequel la substance biologique est séparée des cellules par filtrage. Il est caractérisé en ce qu'au moins 0,001 p / p % de surfactant non ionique est présent dans le milieu de culture des cellules. On a découvert que la présence d'un surfactant non ionique dans le milieu de culture des cellules améliore les performances de filtrage.

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