Method for in vivo detecting target protein gene by drug

Details Method for in vivo detecting target protein gene by drug Method for in vivo detecting target protein gene by drug

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2328719

A method for in vivo detecting a target protein gene by a drug, which comprises binding an antigenic substance to a drug to be administered to a living organism via a chemical crosslinker, using the obtained material as a probe, and directly screening the gene of the protein bonded to the above probe by using a cDNA expression library containing genes of the living organism to which the drug is to be administered. Compared with the conventional drug-fixed column methods, the above method makes it possible to directly and conveniently isolate the gene of the target molecule without resort to the purification of the protein and the analysis of the amino acid sequence thereof.

L'invention concerne un procédé permettant de détecter in vivo un gène protéique cible à l'aide d'un médicament. Ce procédé consiste à fixer une substance antigénique à un médicament devant être administré à un organisme vivant via un réticulant chimique, puis à utiliser le matériau obtenu comme sonde, et à détecter directement le gène de la protéine fixée à la sonde susmentionnée en utilisant une bibliothèque d'expression d'ADNc renfermant des gènes de l'organisme auquel le médicament a été administré. Par rapport aux procédés traditionnels sur colonnes fixées à un médicament, le procédé susmentionné permet d'isoler directement, et de manière pratique, le gène de la molécule cible sans effectuer de purification de la protéine ni d'analyse de sa séquence d'acides aminés.

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