C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C12N 15/10 (2006.01) C12N 15/87 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2308292
Disclosed is a method of producing random polynucleotides by introducing two or more related polynucleotides into a suitable host cell such that a hybrid polynucleotide is generated by recombination and reductive reassortment. Also provided are vector and expression vehicles including such polynucleotides, polypeptides expressed by the hybrid polynucleotides and a method for screening for hybrid polypeptides.
L'invention porte sur un procédé de production de polynucléotides aléatoires consistant à introduire deux ou plus de deux polynucléotides apparentés dans une cellule hôte idoine de manière à former un polynucléotide hybride obtenu par recombinaison et réassortiment réducteur. L'invention porte également sur des vecteurs et véhicules d'expression comportant de tels polynucléotides, sur des polypeptides exprimés par des polynucléotides hybrides, et sur un procédé de criblage des polypeptides hybrides.
Bp Corporation North America Inc.
Diversa Corporation
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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