Glutaryl cephalosporin amidase from pseudomonas diminuta bs-203

Details Glutaryl cephalosporin amidase from pseudomonas diminuta bs-203 Glutaryl cephalosporin amidase from pseudomonas diminuta bs-203

C - Chemistry, Metallurgy

12

N

C12N 9/80 (2006.01) C07D 501/22 (2006.01) C07K 14/21 (2006.01) C12N 5/10 (2006.01) C12N 15/31 (2006.01) C12P 35/02 (2006.01)

Patent

CA 2423161

The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-.alpha.-(4- carboxylbutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.

L'invention concerne du glutaryl 7-ACA amidase provenant de la souche <i>Pseudomonas diminuta</i> <i>BS-203</i>, qui catalyse l'hydrolyse, en acide 7-aminocéphalosporanique et en acide glutarique, de l'acide 7-.alpha.-(4-carboxylbutanamido)-céphalosporanique. L'invention concerne également des séquences d'acide nucléique, des vecteurs et des cellules hôtes utiles dans la production de cette amidase. Le glutaryl 7-ACA amidase peut être utilisé dans la préparation d'acide 7-aminocéphalosporanique.

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