A process for the extraction, purification and enzymatic...

Details A process for the extraction, purification and enzymatic... A process for the extraction, purification and enzymatic...

A - Human Necessities

23

J

A23J 3/16 (2006.01) A23J 1/14 (2006.01) A23L 1/305 (2006.01) A61K 38/16 (2006.01)

Patent

CA 2474106

A process for the extraction, purification and enzymatic modification of .beta.-conglycinin .alpha.' subunit, wherein .beta.-conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the .alpha.' subunit, which is treated with chymotrypsin, then subjected to a further MAC to recover the amino-terminal region of this polypeptide (MW 28,000 Da).

L'invention concerne un procédé d'extraction, de purification et de modification enzymatique de la sous-unité .alpha.' de la .beta.-conglycinine, cette .beta.-conglycinine étant extraite sélectivement de soja moulu et dégraissé, puis précipitée au moyen d'un traitement avec de l'éthanol aqueux. La fraction enrichie est ensuite soumise à une chromatographie d'affinité métallique (MAC) en conditions dénaturantes pour obtenir la sous-unité .alpha.', qui est traitée avec de la chymotrypsine, puis soumise à une nouvelle MAC pour récupérer la région amino-terminale ce de polypeptide (MW 28000 Da).

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