Ezrin assay method for the in vitro diagnosis of colorectal...

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G01N 33/574 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2693648

The invention relates to a method for the in vitro diagnosis of colorectal cancer, by determining the presence of the ezrin tumour marker in a biological sample taken from a patient suspected of having colorectal cancer, using at least one monoclonal anti-ezrin antibody directed against an epitope of ezrin selected from the epitopes having sequences SEQ ID N°l, SEQ ID N°2, SEQ ID N°3, SEQ ID N°4+SEQ ID N°5, SEQ ID N°6+SEQ ID N°7 and SEQ ID N°8. Said method can be used for early diagnosis, screening, therapeutic follow-up and prognosis, as well as for relapse diagnosis in relation to colorectal cancer.

La présente invention concerne un procédé de diagnostic in vitro du cancer colorectal par détermination de la présence du marqueur tumoral Ezrine dans un échantillon biologique issu d'un patient suspecté d'être atteint du cancer colorectal en utilisant au moins un anticorps monoclonal anti-Ezrine dirigé contre un épitope de l'Ezrine choisi parmi les épitopes de séquence SEQ ID N o1, SEQ ID N o2, SEQ ID N o3, SEQ ID N o4+SEQ ID N o5, SEQ ID N o6+SEQ ID N o7 et SEQ ID N o8, ledit procédé pouvant être utilisé tant dans le diagnostic précoce, le dépistage, le suivi thérapeutique, le pronostic, que dans le diagnostic des rechutes dans le cadre du cancer colorectal.

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