Fluid circuit components based upon passive fluid dynamics

B - Operations – Transporting – 81 – B

Patent

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Details

B81B 1/00 (2006.01) B01L 99/00 (2010.01) B01F 5/04 (2006.01) B01F 5/06 (2006.01) B01F 13/00 (2006.01) B01L 3/00 (2006.01) B01L 3/02 (2006.01) F15C 1/14 (2006.01) G01N 33/567 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2347182

Methods of controlling fluid flow through microchannels by use of passive valves or stopping means in the microchannels are presented. The passive valves act as pressure barriers impeding flow of solution past the stopping means until enough force is built up to overcome the force of the pressure barrier. Well planned use of such stopping means acting as passive valves allows the flow of fluids through microchannels to be regulated so as to allow fluids to be mixed or diluted after being introduced via a single channel, or to be split into multiple channels without the need for individual pipetting. Flow through the multiple channels can be regulated to allow a series of sister wells or chambers to all fill prior to the fluid flowing beyond any one of the sister wells or chambers. The filling of sister wells or chambers in this manner allows all wells or chambers to undergo reactions in unison. The use of air ducts to prevent trapping of air in the microchannels is also presented.

L'invention concerne des procédés, permettant de commander l'écoulement d'un fluide dans des microcanaux, au moyen de soupapes passives ou de mécanismes d'arrêt placés dans lesdits microcaneaux. Les soupapes passives agissent comme des barrières anti-pression qui empêchent l'écoulement d'une solution au-delà des mécanismes d'arrêt, jusqu'à ce qu'une force suffisante soit établie afin de vaincre la force de la barrière anti-pression. Une utilisation bien étudiée des mécanismes d'arrêt comme soupapes passives permet de réguler l'écoulement de fluides dans les microcanaux, de sorte que ces fluides peuvent se mélanger ou se diluer après leur introduction dans un canal unique, ou peuvent être séparés dans plusieurs canaux sans avoir besoin de pipettage. Il est possible de réguler l'écoulement dans les différents canaux, de sorte que plusieurs puits ou chambres jumelés peuvent se remplir avant l'écoulement du fluide au-delà de n'importe lequel des puits et des chambres jumelés. Ce type de remplissage permet que tous les puits ou chambres subissent des réactions en même temps. L'invention concerne également l'utilisation de conduits d'air permettant d'empêcher le piégeage d'air dans les microcanaux.

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