Fluorescent organic nanocrystals for producing biosensors

C - Chemistry – Metallurgy – 09 – K

Patent

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C09K 11/02 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2681300

The present invention relates to a (nano)material comprising at least one inorganic and/or organomineral layer, integrated into which is at least one type of organic or organometallic fluorescent nanocrystal that emerges from the surface of said layer so that at least one part of the fluorescent nanocrystals is in direct contact with the outside environment. These emerging nanocrystals constitute, due to their fluorescence, a signalling function, and they may thus be functionalized by grafting a biomolecule bearing a probe function, such as a half-strand of DNA, to thus produce a biochip, biocaptor or biosensor comprising such a (nano)material, a support for a biochip, biocaptor or biosensor, and a process for preparing such a nanomaterial.

La présente invention concerne un (nano) matériau comprenant au moins une couche inorganique et/ou organo- minérale, dans laquelle est intégré au moins un type de nano-cristal fluorescent organique ou organo-métallique émergeant de la surface de ladite couche de sorte qu'au moins une partie des nano-cristaux fluorescents est en contact direct avec le milieu extérieur. Ces nanocristaux émergeants constituant, grâce à leur fluorescence, la fonction de signalisation, peuvent ainsi être fonctionnalisés par greffage d'une biomolécule portant une fonction sonde, tel demi-brin d'ADN, pour réaliser ainsi une bio-puce, capteur ou senseur comprenant un tel (nano) matériau, un support pour bio-puce, capteur ou senseur, et un procédé de préparation d'un tel nanomatériau.

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