G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/573 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2758624
The present invention relates to methods of deter-mining intracellular Cbl-b protein in cells of a sample, comprising .cndot. introducing an antibody, which binds Cbl-b intra-cellularly, into a cell, .cndot. allowing contracting of the antibody and Cbl-b protein potentially present in the cell, .cndot. detecting binding events between the antibody and Cbl-b, .cndot. quantifying the detected binding events, whereby the content of Cbl-b protein is determined.
La présente invention concerne des procédés pour déterminer la protéine Cbl-b intracellulaire dans des cellules d'un échantillon, comprenant l'introduction d'un anticorps qui lie Cbl-b de façon intracellulaire, dans une cellule, la mise en contact de l'anticorps et de protéine Cbl-b potentiellement présente dans la cellule, la détection d'événements de liaison entre l'anticorps et Cbl-b, la quantification des événements de liaison détectés, ce qui permet de déterminer la teneur en protéine Cbl-b.
Baier Gottfried
Gruber Thomas
Lametschwandtner Guenther
Loibner Hans
Schuster Manfred
Apeiron Biologics Ag
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Medizinische Universitaet Innsbruck
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