Method for determining the cbl-b expression

G - Physics – 01 – N

Patent

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G01N 33/573 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2758624

The present invention relates to methods of deter-mining intracellular Cbl-b protein in cells of a sample, comprising .cndot. introducing an antibody, which binds Cbl-b intra-cellularly, into a cell, .cndot. allowing contracting of the antibody and Cbl-b protein potentially present in the cell, .cndot. detecting binding events between the antibody and Cbl-b, .cndot. quantifying the detected binding events, whereby the content of Cbl-b protein is determined.

La présente invention concerne des procédés pour déterminer la protéine Cbl-b intracellulaire dans des cellules d'un échantillon, comprenant l'introduction d'un anticorps qui lie Cbl-b de façon intracellulaire, dans une cellule, la mise en contact de l'anticorps et de protéine Cbl-b potentiellement présente dans la cellule, la détection d'événements de liaison entre l'anticorps et Cbl-b, la quantification des événements de liaison détectés, ce qui permet de déterminer la teneur en protéine Cbl-b.

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