C - Chemistry – Metallurgy – 03 – C
Patent
C - Chemistry, Metallurgy
03
C
C03C 17/34 (2006.01)
Patent
CA 2389579
The present invention is related to a method for obtaining microarrays comprising the steps of: submitting the surface of a solid support to an oxidation of olefinic groups present on said surface in order to allow the formation of aldehyde functions upon the surface of said solid support, covalently binding upon said aldehyde functions capture molecules designed for the detection, the identification, the quantification and/or the recovery of complementary target biological or chemical molecules of interest; said covalent binding resulting in an array comprising a density of at least 4, discrete regions/cm2 of solid support surface, each of said discrete surface regions being bound with a species of capture molecules.
L'invention concerne un procédé d'obtention de microréseaux, qui comporte les étapes consistant à : soumettre la surface d'un support solide à une oxydation de groupes oléfiniques présents sur cette surface afin de permettre la formation de fonctions aldéhyde sur la surface du support solide ; lier de manière covalente sur ces fonctions aldéhyde des molécules de capture conçues pour détecter, identifier, quantifier et/ou extraire des molécules biologiques ou chimiques complémentaires voulues. Cette liaison covalente permet d'obtenir un réseau présentant une densité d'au moins 4 régions distinctes/cm?2¿ de surface de support solide, chacune de ces régions de surface distinctes étant liée à une espèce de molécule de capture.
Hevesi Laszlo
Jeanmart Laurent
Remacle Jose
Facultes Universitaires Notre-Dame de La Paix
Gowling Lafleur Henderson Llp
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