Process for the co-production of chitin, its derivatives and...

Details Process for the co-production of chitin, its derivatives and... Process for the co-production of chitin, its derivatives and...

C - Chemistry, Metallurgy

12

P

C12P 19/26 (2006.01) C12P 19/04 (2006.01)

Patent

CA 2732234

This invention concerns a process for the co-production of glucosamine polymers (chitin, chitosan or any of its derivatives) and polymers containing glucose, mannose and/or galactose, by the high cell density fermentation of the yeast Pichia pastoris in a bioreactor under aerobic conditions, preferably using glycerol byproduct from the biodiesel industry as carbon source. Pure glycerol, pure methanol, glycerol-rich or methanol rich mixtures may also be used as carbon sources. The present invention also concerns P. pastoris fermentation process duly optimized for attaining high cell densities and high cell wall chitin content, as well as polymers containing glucose, mannose and/or galactose.

La présente invention concerne un procédé de co-production de polymères de glucosamine (chitine, chitosane ou n'importe lequel de ses dérivés) et de polymères contenant du glucose, du mannose et/ou du galactose, par la fermentation à haute densité cellulaire de levure Pichia pastoris dans un bioréacteur en conditions aérobies, de préférence au moyen dun sous-produit de glycérol provenant de l'industrie du biodiesel en tant que source de carbone. Du glycérol pur, du méthanol pur, et des mélanges riches en glycérol ou en méthanol peuvent également être utilisés en tant que sources de carbone. La présente invention concerne en outre le processus de fermentation de P. pastoris dûment optimisé pour atteindre de hautes densités cellulaires et une teneur en chitine de paroi cellulaire élevée, ainsi que des polymères contenant du glucose, du mannose et/ou du galactose.

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